* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from HCT116 cells.Using E-Cadherin Rabbit mAb IgG [WY0B] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDH1,CDHE,UVO,CADH1_HUMAN,Cadherin-1 [Cleaved into: E-Cad/CTF1,E-Cad/CTF2,E-Cad/CTF3]
UniProtID :P12830
MASS(da) :97,456
MW(kDa) :135KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human E-Cadherin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HCT116 cells
Function : Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells,cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.., E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.., (Microbial infection) Serves as a receptor for Listeria monocytogenes,internalin A (InlA) binds to this protein and promotes uptake of the bacteria..
Tissue specificity :Non-neural epithelial tissues.
Subcellular locationi :Cell junction, adherens junction. Cell membrane,Single-pass type I membrane protein. Endosome. Golgi apparatus, trans-Golgi network.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.