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Western blot(SDS PAGE) analysis of extracts from C6 cells.Using EEA1 Rabbit mAb IgG [78HI] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EEA1,ZFYVE2,EEA1_HUMAN,Early endosome antigen 1
UniProtID :Q15075
MASS(da) :162,466
MW(kDa) :170kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human EEA1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :C6 cells
Function : Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Subcellular locationi :Cytoplasm. Early endosome membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.