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Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using eEF1G Rabbit mAb IgG [1M7Z] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EEF1G,EF1G,PRO1608,EF1G_HUMAN,Elongation factor 1-gamma
UniProtID :P26641
MASS(da) :50,119
MW(kDa) :48kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human eEF1G.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-29 cells
Function : Probably plays a role in anchoring the complex to other cellular components.
Tissue specificity :Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.