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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using eICC/IF2B epsilon (EICC/IF2B5) Rabbit mAb IgG [7XJ0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EIF2B5,EIF2BE,EI2BE_HUMAN,Translation initiation factor eIF-2B subunit epsilon
UniProtID :Q13144
MASS(da) :80,380
MW(kDa) :80kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human eICC/IF2B epsilon (EICC/IF2B5).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.