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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using eICC/IF4A1 Rabbit mAb IgG [99D8] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EIF4A1,DDX2A,EIF4A,IF4A1_HUMAN,Eukaryotic initiation factor 4A-I
UniProtID :P60842
MASS(da) :46,154
MW(kDa) :46KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human eICC/IF4A1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.