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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using eICC/IF5A Rabbit mAb IgG [YRB8] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :EIF5A,IF5A1_HUMAN,Eukaryotic translation initiation factor 5A-1
UniProtID :P63241
MASS(da) :16,832
MW(kDa) :18kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human eICC/IF5A.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : mRNA-binding protein involved in translation elongation (PubMed:16987817, PubMed:17187778, PubMed:33547280). Has an important function at the level of mRNA turnover, probably acting downstream of decapping (PubMed:16987817, PubMed:17187778, PubMed:33547280). Critical for the efficient synthesis of peptide bonds between consecutive proline residues (PubMed:16987817, PubMed:17187778, PubMed:33547280). Can resolve ribosomal stalling caused by consecutive prolines during translation (PubMed:16987817, PubMed:17187778, PubMed:33547280). Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity (PubMed:16987817, PubMed:17187778, PubMed:33547280). With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis (PubMed:15371445). Regulates also TNF-alpha-mediated apoptosis (PubMed:15452064). Mediates effects of polyamines on neuronal process extension and survival (PubMed:17360499). May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity). Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts (PubMed:8253832)..
Tissue specificity :Expressed in umbilical vein endothelial cells and several cancer cell lines (at protein level)..
Subcellular locationi :Cytoplasm. Nucleus. Endoplasmic reticulum membrane,Peripheral membrane protein,Cytoplasmic side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.