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Western blot(SDS PAGE) analysis of extracts from .Using ENT2/SLC29A2 Rabbit mAb IgG [9MP5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :SLC29A2,DER12,ENT2,HNP36,S29A2_HUMAN,Equilibrative nucleoside transporter 2
UniProtID :Q14542
MASS(da) :50,113
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ENT2/SLC29A2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
Function : Mediates equilibrative transport of purine, pyrimidine nucleosides and the purine base hypoxanthine. Very less sensitive than SLC29A1 to inhibition by nitrobenzylthioinosine (NBMPR), dipyridamole, dilazep and draflazine..
Tissue specificity :Expressed in skeletal muscle, liver, lung, placenta, brain, heart, kidney and ovarian tissues..
Subcellular locationi :Basolateral cell membrane,Multi-pass membrane protein. Nucleus membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.