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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using Erlin-2 Rabbit mAb IgG [XK63] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ERLIN2,C8orf2,SPFH2,UNQ2441/PRO5003/PRO9924,ERLN2_HUMAN,Erlin-2
UniProtID :O94905
MASS(da) :37,840
MW(kDa) :43KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Erlin-2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs) such as ITPR1 (PubMed:19240031, PubMed:17502376). Promotes sterol-accelerated ERAD of HMGCR probably implicating an AMFR/gp78-containing ubiquitin ligase complex (PubMed:21343306). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. May promote ER retention of the SCAP-SREBF complex (PubMed:24217618)..
Tissue specificity :Ubiquitous..
Subcellular locationi :Endoplasmic reticulum membrane,Single-pass type II membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.