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Western blot(SDS PAGE) analysis of extracts from Mouse skeletal muscle tissue lysate.Using Fbx32/FBOX32 Rabbit mAb IgG [CX31] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FBXO32,FBX32_HUMAN,F-box only protein 32
UniProtID :Q969P5
MASS(da) :41,637
MW(kDa) :42kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Mouse Fbx32/FBOX32.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse skeletal muscle tissue lysate
Function : Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1..
Tissue specificity :Specifically expressed in cardiac and skeletal muscle.
Subcellular locationi :Cytoplasm. Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.