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Folate Binding Protein(FBP) / FOLR1 Rabbit mAb[5Z1Y]Cat NO.: A25808

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Folate Binding Protein(FBP) / FOLR1 Rabbit mAb IgG [5Z1Y] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :FOLR1,FOLR,FOLR1_HUMAN,Folate receptor alpha

UniProtID :P15328

MASS(da) :29,819

MW(kDa) :38KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Folate Binding Protein(FBP) / FOLR1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728, PubMed:19074442). Has high affinity for folate and folic acid analogs at neutral pH (PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed:8567728). Required for normal embryonic development and normal cell proliferation (By similarity)..

Tissue specificity :Primarily expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. Detected in placenta and thymus epithelium..

Subcellular locationi :Cell membrane,Lipid-anchor, GPI-anchor. Apical cell membrane,Lipid-anchor, GPI-anchor. Basolateral cell membrane,Lipid-anchor, GPI-like-anchor. Secreted. Cytoplasmic vesicle. Cytoplasmic vesicle, clathrin-coated vesicle. Endosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.