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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using GABA transaminase (ABAT) Rabbit mAb IgG [2GU0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ABAT,GABAT,GABT_HUMAN,4-aminobutyrate aminotransferase, mitochondrial -3-amino-2-methylpropionate transaminase)
UniProtID :P80404
MASS(da) :56,439
MW(kDa) :50KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human GABA transaminase (ABAT).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively (PubMed:10407778, PubMed:15528998). Can also convert delta-aminovalerate and beta-alanine (By similarity)..
Tissue specificity :Liver > pancreas > brain > kidney > heart > placenta..
Subcellular locationi :Mitochondrion matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.