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Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using Galectin 3/LGALS3 Rabbit mAb IgG [RKH6] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LGALS3,MAC2,LEG3_HUMAN,Galectin-3
UniProtID :P17931
MASS(da) :26,152
MW(kDa) :28KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Galectin 3/LGALS3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-29 cells
Function : Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes..
Tissue specificity :A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes..
Subcellular locationi :Cytoplasm. Nucleus. Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.