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Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using Galectin 4/LGALS4 Rabbit mAb IgG [YJ3M] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LGALS4,LEG4_HUMAN,Galectin-4
UniProtID :P56470
MASS(da) :35,941
MW(kDa) :36kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Galectin 4/LGALS4.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-29 cells
Function : Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.