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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using Gephyrin Rabbit mAb IgG [445Q] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GPHN,GPH,KIAA1385,GEPH_HUMAN,Gephyrin [Includes: Molybdopterin adenylyltransferase ,Molybdopterin molybdenumtransferase ]
UniProtID :Q9NQX3
MASS(da) :79,748
MW(kDa) :93kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Gephyrin.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Microtubule-associated protein involved in membrane protein-cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (By similarity). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors (PubMed:25025157, PubMed:26613940).., Has also a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released..
Subcellular locationi :Cell junction, synapse, postsynaptic cell membrane,Lipid-anchor,Cytoplasmic side. Cell membrane,Lipid-anchor,Cytoplasmic side. Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Cell projection, dendrite. Cell junction, synapse, postsynaptic density.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.