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GFAP Rabbit mAb[A00L]Cat NO.: A23755

Western blot(SDS PAGE) analysis of extracts from Mouse brain tissue lysate.Using GFAP Rabbit mAb IgG [A00L] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :GFAP,GFAP_HUMAN,Glial fibrillary acidic protein

UniProtID :P14136

MASS(da) :49,880

MW(kDa) :50kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human GFAP.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Mouse brain tissue lysate

Function : GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Tissue specificity :Expressed in cells lacking fibronectin..

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.