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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using GTF2F2 Rabbit mAb IgG [I4D3] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GTF2F2,RAP30,T2FB_HUMAN,General transcription factor IIF subunit 2
UniProtID :P13984
MASS(da) :28,380
MW(kDa) :30KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human GTF2F2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.