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Western blot(SDS PAGE) analysis of extracts from Rat brain tissue lysate.Using HAPLN1 Rabbit mAb IgG [36V8] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HAPLN1,CRTL1,HPLN1_HUMAN,Hyaluronan and proteoglycan link protein 1
UniProtID :P10915
MASS(da) :40,166
MW(kDa) :57kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HAPLN1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Rat brain tissue lysate
Function : Stabilizes the aggregates of proteoglycan monomers with hyaluronic acid in the extracellular cartilage matrix.
Tissue specificity :Widely expressed. Weakly expressed in the brain..
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.