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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using HFE Rabbit mAb IgG [0KY4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HFE,HLAH,HFE_HUMAN,Hereditary hemochromatosis protein
UniProtID :Q30201
MASS(da) :40,108
MW(kDa) :45kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HFE.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin..
Tissue specificity :Expressed in all tissues tested except brain.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.