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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using HLTF Rabbit mAb IgG [CFSG] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HLTF,HIP116A,RNF80,SMARCA3,SNF2L3,ZBU1,HLTF_HUMAN,Helicase-like transcription factor
UniProtID :Q14527
MASS(da) :113,929
MW(kDa) :120kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HLTF.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity,This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA..
Tissue specificity :Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle..
Subcellular locationi :Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.