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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Hsc70-interacting protein (HIP) Rabbit mAb IgG [323G] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ST13,AAG2,FAM10A1,HIP,SNC6,F10A1_HUMAN,Hsc70-interacting protein
UniProtID :P50502
MASS(da) :41,332
MW(kDa) :47kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Hsc70-interacting protein (HIP).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins (By similarity)..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.