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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using HSPA14 Rabbit mAb IgG [OJG2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HSPA14,HSP60,HSP70L1,HSP7E_HUMAN,Heat shock 70 kDa protein 14
UniProtID :Q0VDF9
MASS(da) :54,794
MW(kDa) :55kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HSPA14.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity..
Subcellular locationi :Cytoplasm, cytosol.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.