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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using HTRA2 Rabbit mAb IgG [6S8C] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :HTRA2,OMI,PRSS25,HTRA2_HUMAN,Serine protease HTRA2, mitochondrial
UniProtID :O43464
MASS(da) :48,841
MW(kDa) :38kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human HTRA2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive..
Tissue specificity :[Isoform 1]: Ubiquitously expressed..
Subcellular locationi :Mitochondrion intermembrane space. Mitochondrion membrane,Single-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.