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ICAM3 / CD50 Rabbit mAb[079V]Cat NO.: A38370

Western blot(SDS PAGE) analysis of extracts from U-937 cells.Using ICAM3 / CD50 Rabbit mAb IgG [079V] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :ICAM3,ICAM3_HUMAN,Intercellular adhesion molecule 3

UniProtID :P32942

MASS(da) :59,541

MW(kDa) :120-140KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ICAM3 / CD50.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :U-937 cells

Function : ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590)..

Tissue specificity :Leukocytes..

Subcellular locationi :Membrane,Single-pass type I membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.