* 产品详情- */>
Western blot(SDS PAGE) analysis of extracts from U-937 cells.Using ICAM3 / CD50 Rabbit mAb IgG [079V] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ICAM3,ICAM3_HUMAN,Intercellular adhesion molecule 3
UniProtID :P32942
MASS(da) :59,541
MW(kDa) :120-140KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human ICAM3 / CD50.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U-937 cells
Function : ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590)..
Tissue specificity :Leukocytes..
Subcellular locationi :Membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.