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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using IGFBP1 Rabbit mAb IgG [U8Y4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :IGFBP1,IBP1,IBP1_HUMAN,Insulin-like growth factor-binding protein 1
UniProtID :P08833
MASS(da) :27,904
MW(kDa) :30kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human IGFBP1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.