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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using IQGAP2 Rabbit mAb IgG [6ZA2] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :IQGAP2,IQGA2_HUMAN,Ras GTPase-activating-like protein IQGAP2
UniProtID :Q13576
MASS(da) :180,578
MW(kDa) :180KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human IQGAP2.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.
Tissue specificity :Isoform 2 expression is enhanced in testis.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.