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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using Kininogen 1 (KNG1) Rabbit mAb IgG [LTF5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :KNG1,BDK,KNG,KNG1_HUMAN,Kininogen-1 [Cleaved into: Kininogen-1 heavy chain,T-kinin ,Bradykinin ,Lysyl-bradykinin ,Kininogen-1 light chain,Low molecular weight growth-promoting factor]
UniProtID :P01042
MASS(da) :71,957
MW(kDa) :46kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Kininogen 1 (KNG1).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : (1) Kininogens are inhibitors of thiol proteases,(2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII,(3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes,(4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action),(5) LMW-kininogen inhibits the aggregation of thrombocytes,(6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
Tissue specificity :Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors..
Subcellular locationi :Secreted, extracellular space.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.