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Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using KRAS+HRAS+NRAS Rabbit mAb IgG [EQUL] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NRAS,HRAS1,RASN_HUMAN,GTPase NRas
UniProtID :P01111/P01112/P01116
MASS(da) :21,656
MW(kDa) :21KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human KRAS+HRAS+NRAS.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HT-29 cells
Function : Ras proteins bind GDP/GTP and possess intrinsic GTPase activity..
Subcellular locationi :Cell membrane,Lipid-anchor,Cytoplasmic side. Golgi apparatus membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.