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LDHA Rabbit mAb[PU7V]Cat NO.: A74345

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using LDHA Rabbit mAb IgG [PU7V] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :LDHA,PIG19,LDHA_HUMAN,L-lactate dehydrogenase A chain

UniProtID :P00338

MASS(da) :36,689

MW(kDa) :37kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human LDHA.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+)..

Tissue specificity :Predominantly expressed in anaerobic tissues such as skeletal muscle and liver..

Subcellular locationi :Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.