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LEFTY1/2 Rabbit mAb[ZK4S]Cat NO.: A86252

Western blot(SDS PAGE) analysis of extracts from HT-29 cells.Using LEFTY1/2 Rabbit mAb IgG [ZK4S] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :LEFTY1,LEFTB,LEFTYB,UNQ278/PRO317,LFTY1_HUMAN,Left-right determination factor 1

UniProtID :O75610/O00292

MASS(da) :40,920

MW(kDa) :41KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human LEFTY1/2.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HT-29 cells

Function : Required for left-right axis determination as a regulator of LEFTY2 and NODAL.

Subcellular locationi :Secreted.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.