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Western blot(SDS PAGE) analysis of extracts from A-549 cells.Using MAD3/MXD3 Rabbit mAb IgG [T7Q4] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MXD3,BHLHC13,MAD3,MAD3_HUMAN,Max dimerization protein 3
UniProtID :Q9BW11
MASS(da) :23,477
MW(kDa) :24kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MAD3/MXD3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A-549 cells
Function : Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation (By similarity)..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.