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Western blot(SDS PAGE) analysis of extracts from A-549 cells.Using Mast Cell Chymase (CMA1) Rabbit mAb IgG [0A74] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CMA1,CYH,CYM,CMA1_HUMAN,Chymase
UniProtID :P23946
MASS(da) :27,325
MW(kDa) :27KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Mast Cell Chymase (CMA1).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A-549 cells
Function : Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Tissue specificity :Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions..
Subcellular locationi :Secreted. Cytoplasmic granule.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.