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Western blot(SDS PAGE) analysis of extracts from PC-12 cells.Using MC5 Receptor Rabbit mAb IgG [FWP8] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MC5R,MC5R_HUMAN,Melanocortin receptor 5
UniProtID :P33032
MASS(da) :36,601
MW(kDa) :50KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MC5 Receptor.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :PC-12 cells
Function : Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. This receptor is a possible mediator of the immunomodulation properties of melanocortins.
Tissue specificity :Expressed in the brain but not in the melanoma cells.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.