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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using MEKK3 Rabbit mAb IgG [FNBB] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MAP3K3,MAPKKK3,MEKK3,M3K3_HUMAN,Mitogen-activated protein kinase kinase kinase 3
UniProtID :Q99759
MASS(da) :70,898
MW(kDa) :78kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MEKK3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.