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MUTA Rabbit mAb[055Q]Cat NO.: A68177

Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using MUTA Rabbit mAb IgG [055Q] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :MMUT,MUT,MUTA_HUMAN,Methylmalonyl-CoA mutase, mitochondrial

UniProtID :P22033

MASS(da) :83,134

MW(kDa) :83KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MUTA.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cells

Function : Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle..

Subcellular locationi :Mitochondrion matrix. Mitochondrion. Cytoplasm.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.