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Western blot(SDS PAGE) analysis of extracts from HepG2 cells.Using MVK Rabbit mAb IgG [353N] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MVK,KIME_HUMAN,Mevalonate kinase
UniProtID :Q03426
MASS(da) :42,451
MW(kDa) :42KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MVK.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cells
Function : Catalyzes the phosphorylation of mevalonate to mevalonate 5-phosphate, a key step in isoprenoid and cholesterol biosynthesis (PubMed:9325256, PubMed:18302342, PubMed:9392419, PubMed:11278915)..
Subcellular locationi :Cytoplasm. Peroxisome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.