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MYL12B Rabbit mAb[57K3]Cat NO.: A38597

Western blot(SDS PAGE) analysis of extracts from K-562 cells.Using MYL12B Rabbit mAb IgG [57K3] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :MYL12B,MRLC2,MYLC2B,ML12B_HUMAN,Myosin regulatory light chain 12B

UniProtID :O14950

MASS(da) :19,779

MW(kDa) :20KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MYL12B.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :K-562 cells

Function : Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion..

Tissue specificity :Ubiquitously expressed in various hematopoietic cells..

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.