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Western blot(SDS PAGE) analysis of extracts from K-562 cells.Using MYL12B Rabbit mAb IgG [57K3] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MYL12B,MRLC2,MYLC2B,ML12B_HUMAN,Myosin regulatory light chain 12B
UniProtID :O14950
MASS(da) :19,779
MW(kDa) :20KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human MYL12B.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K-562 cells
Function : Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion..
Tissue specificity :Ubiquitously expressed in various hematopoietic cells..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.