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Myosin heavy chain Rabbit mAb[B1BB]Cat NO.: A47125

Western blot(SDS PAGE) analysis of extracts from Mouse lung tissue lysate.Using Myosin heavy chain Rabbit mAb IgG [B1BB] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :MYH7,MYHCB,MYH7_HUMAN,Myosin-7

UniProtID :P12883/P13533

MASS(da) :223,735

MW(kDa) :224kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Mouse Myosin heavy chain.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Mouse lung tissue lysate

Function : Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle..

Tissue specificity :Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level)..

Subcellular locationi :Cytoplasm, myofibril. Cytoplasm, myofibril, sarcomere.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.