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NCF4/p40-phox Rabbit mAb[ESR5]Cat NO.: A65706

Western blot(SDS PAGE) analysis of extracts from THP-1 cells.Using NCF4/p40-phox Rabbit mAb IgG [ESR5] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NCF4,SH3PXD4,NCF4_HUMAN,Neutrophil cytosol factor 4

UniProtID :Q15080

MASS(da) :39,032

MW(kDa) :40KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NCF4/p40-phox.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :THP-1 cells

Function : Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex..

Tissue specificity :Expression is restricted to hematopoietic cells.

Subcellular locationi :Cytoplasm, cytosol. Endosome membrane,Peripheral membrane protein,Cytoplasmic side. Membrane,Peripheral membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.