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Western blot(SDS PAGE) analysis of extracts from U2OS cells.Using NDUFAF1 Rabbit mAb IgG [574M] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NDUFAF1,CIA30,CGI-65,CIA30_HUMAN,Complex I intermediate-associated protein 30, mitochondrial
UniProtID :Q9Y375
MASS(da) :37,764
MW(kDa) :38KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NDUFAF1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U2OS cells
Function : As part of the MCIA complex, involved in the assembly of the mitochondrial complex I..
Tissue specificity :Ubiquitous..
Subcellular locationi :Mitochondrion. Mitochondrion matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.