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NDUFAF1 Rabbit mAb[574M]Cat NO.: A95248

Western blot(SDS PAGE) analysis of extracts from U2OS cells.Using NDUFAF1 Rabbit mAb IgG [574M] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NDUFAF1,CIA30,CGI-65,CIA30_HUMAN,Complex I intermediate-associated protein 30, mitochondrial

UniProtID :Q9Y375

MASS(da) :37,764

MW(kDa) :38KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NDUFAF1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :U2OS cells

Function : As part of the MCIA complex, involved in the assembly of the mitochondrial complex I..

Tissue specificity :Ubiquitous..

Subcellular locationi :Mitochondrion. Mitochondrion matrix.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.