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NDUFB8 Rabbit mAb[K118]Cat NO.: A16637

Western blot(SDS PAGE) analysis of extracts from Rat heart tissue lysate.Using NDUFB8 Rabbit mAb IgG [K118] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NDUFB8,NDUB8_HUMAN,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

UniProtID :O95169

MASS(da) :21,766

MW(kDa) :22kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NDUFB8.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Rat heart tissue lysate

Function : Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone..

Subcellular locationi :Mitochondrion inner membrane,Single-pass membrane protein,Matrix side.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.