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Neurofilament M Rabbit mAb[1KZ9]Cat NO.: A11736

Western blot(SDS PAGE) analysis of extracts from 293T cells.Using Neurofilament M Rabbit mAb IgG [1KZ9] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NEFM,NEF3,NFM,NFM_HUMAN,Neurofilament medium polypeptide

UniProtID :P07197

MASS(da) :102,472

MW(kDa) :160kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Neurofilament M.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :293T cells

Function : Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity)..

Subcellular locationi :Cytoplasm, cytoskeleton. Cell projection, axon.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.