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Western blot(SDS PAGE) analysis of extracts from 293T cells.Using Neurofilament M Rabbit mAb IgG [1KZ9] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NEFM,NEF3,NFM,NFM_HUMAN,Neurofilament medium polypeptide
UniProtID :P07197
MASS(da) :102,472
MW(kDa) :160kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Neurofilament M.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293T cells
Function : Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity)..
Subcellular locationi :Cytoplasm, cytoskeleton. Cell projection, axon.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.