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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using Neurokinin 1 Receptor (NK1R) Rabbit mAb IgG [X6R0] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :TACR1,NK1R,TAC1R,NK1R_HUMAN,Substance-P receptor
UniProtID :P25103
MASS(da) :46,251
MW(kDa) :48kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Neurokinin 1 Receptor (NK1R).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.