support:13376277882 | tech@naturebios.com

Neutrophil Elastase Rabbit mAb[1LFQ]Cat NO.: A46988

Western blot(SDS PAGE) analysis of extracts from THP-1 cells.Using Neutrophil Elastase (ELANE) Rabbit mAb IgG [1LFQ] at dilution of 1:1000 incubated at 4℃ over night.

  • SizePriceIn stock
  • 50ul1280咨询客服
  • 100ul2380咨询客服
Order Datasheet

Product information

Protein names :ELANE,ELA2,ELNE_HUMAN,Neutrophil elastase

UniProtID :P08246

MASS(da) :28,518

MW(kDa) :30KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Neutrophil Elastase (ELANE).

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :THP-1 cells

Function : Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro,digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984)..

Tissue specificity :Bone marrow cells. Neutrophil (PubMed:10947984)..

Subcellular locationi :Cytoplasmic vesicle, phagosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.