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Western blot(SDS PAGE) analysis of extracts from THP-1 cells.Using Neutrophil Elastase (ELANE) Rabbit mAb IgG [1LFQ] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ELANE,ELA2,ELNE_HUMAN,Neutrophil elastase
UniProtID :P08246
MASS(da) :28,518
MW(kDa) :30KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Neutrophil Elastase (ELANE).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :THP-1 cells
Function : Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro,digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984)..
Tissue specificity :Bone marrow cells. Neutrophil (PubMed:10947984)..
Subcellular locationi :Cytoplasmic vesicle, phagosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.