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Western blot(SDS PAGE) analysis of extracts from Mouse kidney tissue lysate.Using NME2/NM23B Rabbit mAb IgG [09AI] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NME2,NM23B,NDKB_HUMAN,Nucleoside diphosphate kinase B
UniProtID :P22392
MASS(da) :17,298
MW(kDa) :17kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of Mouse NME2/NM23B.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Mouse kidney tissue lysate
Function : Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity). Negatively regulates Rho activity by interacting with AKAP13/LBC (PubMed:15249197). Acts as a transcriptional activator of the MYC gene,binds DNA non-specifically (PubMed:8392752, PubMed:19435876). Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III(1) region of the MYC gene promoter. Does not bind to duplex NHE III(1) (PubMed:19435876). Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds both folded and unfolded G4 with similar low nanomolar affinities. Stabilizes folded G4s regardless of whether they are prefolded or not (PubMed:25679041). Exhibits histidine protein kinase activity (PubMed:20946858)..
Tissue specificity :[Isoform 1]: Ubiquitously expressed..,[Isoform 3]: Ubiquitously expressed..
Subcellular locationi :Cytoplasm. Cell projection, lamellipodium. Cell projection, ruffle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.