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NRAS Rabbit mAb[DC6D]Cat NO.: A23932

Western blot(SDS PAGE) analysis of extracts from Rat lung tissue lysate.Using NRAS Rabbit mAb IgG [DC6D] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NRAS,HRAS1,RASN_HUMAN,GTPase NRas

UniProtID :P01111/P01112/P01116_

MW(kDa) :20KDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000,
  • 免疫荧光(ICC/IF) 1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NRAS.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Rat lung tissue lysate

Function : Ras proteins bind GDP/GTP and possess intrinsic GTPase activity..

Subcellular locationi :Cell membrane,Lipid-anchor,Cytoplasmic side. Golgi apparatus membrane,Lipid-anchor.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.