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Western blot(SDS PAGE) analysis of extracts from NIH/3T3 cells.Using NRF1 Rabbit mAb IgG [UAEU] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NRF1,NRF1_HUMAN,Nuclear respiratory factor 1
UniProtID :Q16656
MASS(da) :53,541
MW(kDa) :68KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NRF1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :NIH/3T3 cells
Function : Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
Tissue specificity :Ubiquitously expressed with strongest expression in skeletal muscle.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.