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NSD3/WHSC1L1 Rabbit mAb[H09D]Cat NO.: A27546

Western blot(SDS PAGE) analysis of extracts from Mouse brain tissue lysate.Using NSD3/WHSC1L1 Rabbit mAb IgG [H09D] at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :NSD3,WHSC1L1,DC28,NSD3_HUMAN,Histone-lysine N-methyltransferase NSD3

UniProtID :Q9BZ95

MASS(da) :161,613

MW(kDa) :200kDa

Form :Liquid

Purification :Protein A purification

Host :Rabbit

Isotype :IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human NSD3/WHSC1L1.

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Mouse brain tissue lysate

Function : Histone methyltransferase. Preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3 forming H3K2me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression..

Tissue specificity :Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung..

Subcellular locationi :Nucleus. Chromosome.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.