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Western blot(SDS PAGE) analysis of extracts from U-251MG cells.Using Olig3 Rabbit mAb IgG [FN75] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :OLIG3,BHLHB7,BHLHE20,OLIG3_HUMAN,Oligodendrocyte transcription factor 3
UniProtID :Q7RTU3
MASS(da) :29,358
MW(kDa) :29kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Olig3.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U-251MG cells
Function : May determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.