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Western blot(SDS PAGE) analysis of extracts from A-549 cells.Using Osteocalcin (BGLAP) Rabbit mAb IgG [N2H7] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BGLAP,OSTCN_HUMAN,Osteocalcin
UniProtID :P02818
MASS(da) :10,963
MW(kDa) :11kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human Osteocalcin (BGLAP).
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A-549 cells
Function : Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.