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Western blot(SDS PAGE) analysis of extracts from HeLa cells.Using p60 CAF1 Rabbit mAb IgG [DIP5] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CHAF1B,CAF1A,CAF1P60,MPHOSPH7,MPP7,CAF1B_HUMAN,Chromatin assembly factor 1 subunit B
UniProtID :Q13112
MASS(da) :61,493
MW(kDa) :70KDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with a synthetic peptide of human p60 CAF1.
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells
Function : Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA,histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer..
Subcellular locationi :Nucleus. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.